| US 7,611,860 B2 | ||
| Indicator for in-situ detecting of lysozyme | ||
| Drew M Ferguson, Cambridge (United Kingdom); Guy D. Milan, Great Shelford (United Kingdom); Crawford S. Dow, Coventry (United Kingdom); and Uthaya Swoboda, Coventry (United Kingdom) | ||
| Assigned to Cambridge Meditech Limited, Cambridge (United Kingdom) | ||
| Appl. No. 10/381,426 PCT Filed Oct. 15, 2001, PCT No. PCT/GB01/04588 § 371(c)(1), (2), (4) Date Jul. 15, 2003, PCT Pub. No. WO02/30478, PCT Pub. Date Apr. 18, 2002. |
||
| Prior Publication US 2004/0043422 A1, Mar. 04, 2004 | ||
| Int. Cl. C08L 5/08 (2006.01); G01N 33/53 (2006.01); C12Q 1/34 (2006.01) | ||
| U.S. Cl. 435—18 [435/7.2; 106/162.1] | 28 Claims |
| 1. An indicator suitable for detecting at a location the presence of lysozyme or the presence of nicotinamide adenine dinucleotide
(NADH) in the presence of lysozyme, wherein the indicator comprises:
(a) a biopolymer layer which is susceptible to degradation by lysozyme; and
(b) a signaling layer which comprises an indicator substance, which layer is adapted to produce a detectable signal upon contact
with lysozyme or upon contact with NADH in the presence of lysozyme;
wherein, in use, the signaling layer (b) is at least initially protected from contact with NADH by the layer (a), and wherein
the signaling layer (b) is adapted to produce a detectable signal whose initial strength is proportional to the amount of
NADH or lysozyme, and wherein the signaling layer (b) further comprises a pH indicator or assay reagents for detecting an
additional enzyme, an immune cell or a micro-organism.
|