| US 7,611,854 B2 | ||
| Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor | ||
| Dana M. Fowlkes, Chapel Hill, N.C. (US); Jim Broach, Princeton, N.J. (US); John Manfredi, Ossining, N.Y. (US); Christine Klein, Ossining, N.Y. (US); Andrew J. Murphy, Montclair, N.J. (US); Jeremy Paul, South Nyack, N.Y. (US); and Joshua Trueheart, South Nyack, N.Y. (US) | ||
| Assigned to Cadus Technologies, Inc., New York, N.Y. (US) | ||
| Filed on Jun. 18, 2003, as Appl. No. 10/600,003. | ||
| Application 10/600003 is a continuation of application No. 09/286166, filed on Apr. 05, 1999, granted, now 7,416,881. | ||
| Application 09/286166 is a continuation in part of application No. 08/322137, filed on Oct. 13, 1994, granted, now 6,100,042. | ||
| Application 08/322137 is a continuation in part of application No. 08/309313, filed on Sep. 20, 1994, abandoned. | ||
| Application 08/309313 is a continuation in part of application No. 08/190328, filed on Jan. 31, 1994, abandoned. | ||
| Application 08/190328 is a continuation in part of application No. 08/041431, filed on Mar. 31, 1993, abandoned. | ||
| Prior Publication US 2004/0197840 A1, Oct. 07, 2004 | ||
| Int. Cl. C12N 1/19 (2006.01); G01N 33/53 (2006.01) | ||
| U.S. Cl. 435—7.31 [435/254.2] | 10 Claims |
| 1. A method of detecting ligand binding to a heterologous G protein coupled receptor comprising:
a) providing transformed yeast cells comprising a reporter gene under control of a pheromone-responsive promoter, a heterologous
G protein-coupled receptor gene, each said gene being under the control of a separate promoter, a mutation in a SCG1/GPA1
gene, and a hybrid G.alpha. protein;
b) combining said cells with a compound to be tested; and
c) detecting if said compound binds to said heterologous G protein coupled receptor.
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