| US 7,611,621 B2 | ||
| Disposable oxygen sensor and method for correcting oxygen effect on oxidase-based analytical devices | ||
| Xiaohua Cai, Needham, Mass. (US); Kara Alesi, Winchester, Mass. (US); and Chung Chang Young, Weston, Mass. (US) | ||
| Assigned to Nova Biomedical Corporation, Waltham, Mass. (US) | ||
| Filed on Jun. 13, 2005, as Appl. No. 11/160,193. | ||
| Prior Publication US 2006/0278537 A1, Dec. 14, 2006 | ||
| Int. Cl. G01N 27/327 (2006.01); C12Q 1/26 (2006.01); C12Q 1/28 (2006.01); C12Q 1/54 (2006.01) | ||
| U.S. Cl. 205—777.5 [204/403.04; 204/403.01; 205/782; 205/792] | 33 Claims |
| 1. A method of correcting the oxygen effect on oxidase-based analyte sensors comprising:
determining an oxygen concentration of a fluid sample using an oxygen sensor comprising a working electrode, a reference electrode
and a reagent matrix disposed on at least said working electrode wherein said reagent matrix contains a reduced form of a
redox mediator, an oxidase and a peroxidase and wherein said oxygen sensor has a known correlation between oxygen concentration
and analyte concentration expressed as a function of the response of said oxygen sensor to both oxygen and an analyte wherein
said analyte is a substrate of said oxidase;
using an oxidased-based analyte sensor wherein the correlation between oxygen concentration and oxygen effect on said oxidase-based
analyte sensor is known and wherein an oxidase in said oxidased-based sensor is a substrate of said oxidase in said oxygen
sensor;
determining an analyte concentration of said fluid sample using said oxidase-based analyte sensor; and
calculating a corrected analyte concentration in said fluid sample using said determined oxygen concentration.
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