| US 7,604,973 B2 | ||
| DNA encoding hyaluronan synthase from Pasteurella multocida and methods of use | ||
| Paul DeAngelis, Edmond, Okla. (US) | ||
| Assigned to The Board of Regents of the University of Oklahoma, Norman, Okla. (US) | ||
| Filed on May 01, 2007, as Appl. No. 11/799,130. | ||
| Application 11/799130 is a continuation of application No. 11/124215, filed on May 09, 2005, granted, now 7,232,684. | ||
| Application 11/124215 is a continuation of application No. 10/217613, filed on Aug. 12, 2002, granted, now 6,987,023, filed on Jan. 17, 2006. | ||
| Application 10/217613 is a continuation of application No. 09/283402, filed on Apr. 01, 1999, abandoned. | ||
| Application 09/283402 is a continuation in part of application No. 09/178851, filed on Oct. 26, 1998, abandoned. | ||
| Claims priority of provisional application 60/080414, filed on Apr. 02, 1998. | ||
| Prior Publication US 2007/0298461 A1, Dec. 27, 2007 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. C12N 9/10 (2006.01); C12N 15/00 (2006.01); C12Q 1/68 (2006.01) | ||
| U.S. Cl. 435—193 [435/320.1; 435/252.31; 435/6] | 22 Claims |
| 1. A method for producing hyaluronic acid, comprising the steps of:
introducing a purified nucleic acid segment having a coding region encoding enzymatically active hyaluronan synthase from
Pasteurella into a host organism, wherein the enzymatically active hyaluronan synthase is a single protein that polymerizes UDP-GlcA and
UDP-GlcNAc to form hyaluronic acid, and wherein the nucleic acid segment is selected from the group consisting of:
(a) the nucleic acid sequence as set forth in SEQ ID NO:2;
(b) a nucleic acid segment encoding SEQ ID NO:1; and
(c) a nucleic acid segment encoding SEQ ID NO:17;
growing the host organism in a medium to secrete hyaluronic acid; and
recovering the secreted hyaluronic acid.
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