| US 7,604,798 B2 | ||
| Methods and compositions for importing nucleic acids into cell nuclei | ||
| David A. Dean, Oak Park, Ill. (US) | ||
| Assigned to Northwestern University, Evanston, Ill. (US) | ||
| Filed on Jul. 15, 2005, as Appl. No. 11/182,933. | ||
| Claims priority of provisional application 60/588206, filed on Jul. 15, 2004. | ||
| Prior Publication US 2006/0070133 A1, Mar. 30, 2006 | ||
| Int. Cl. A01N 63/00 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01); C12Q 1/70 (2006.01) | ||
| U.S. Cl. 424—93.21 [536/23.1; 435/5] | 5 Claims |
| 1. A method for tissue specific nuclear import, comprising:
a) providing
i) a construct comprising a cell-specific nuclear targeting signal operably linked to a nucleic acid of interest, wherein
said cell-specific nuclear targeting signal comprises at least nucleotides 149 to 337 of the surfactant protein C(SPC) promoter
region; shown in SEQ ID NO:1 that functions as a nuclear targeting signal; and
ii) a non-dividing mouse or human alveolar type II cell in vitro; and
b) contacting said cell with said construct under conditions such that said nucleic acid of interest is expressed in the nucleus
of said cell.
|