| US 7,601,506 B2 | ||
| Identification of leucocytes bearing diagnostic markers for transmissible spongiform encephalopathies | ||
| Roy Jackman, Derbyshire (United Kingdom); Linda Ann Terry, Berkshire (United Kingdom); and Sally Jane Everest, Surrey (United Kingdom) | ||
| Assigned to IDEXX Laboratories, Inc., Westbrook, Me. (US) | ||
| Filed on Jul. 28, 2006, as Appl. No. 11/494,964. | ||
| Prior Publication US 2008/0026364 A1, Jan. 31, 2008 | ||
| Int. Cl. G01N 33/53 (2006.01) | ||
| U.S. Cl. 435—7.1 [436/501; 436/536; 436/513; 436/547] | 6 Claims |
| 1. A method for detecting a transmissible spongiform encephalopathy (TSE) agent comprising:
(a) contacting isolated leucocytes from a subject with an antibody selective for PrPSc, wherein the antibody is produced by hybridoma ATCC PTA-7393;
(b) contacting the isolated leucocytes with anti-immunoglobulin coated beads;
(c) examining the beads and leucocytes for a rosetting effect;
wherein a rosetting effect indicates detection of the TSE agent.
|