US 7,598,040 B2
	Compositions and methods for the identification of inhibitors of protein synthesis
Richard H. Guenther, Cary, N.C. (US); Winnell H. Newman, Cary, N.C. (US); Samuel P. Yenne, Cary, N.C. (US); Dan Mitchell, Raleigh, N.C. (US); and Andrzej Malkiewicz, Lodz (Poland)
Assigned to Trana Discovery, Inc., Cary, N.C. (US)
Filed on Nov. 21, 2007, as Appl. No. 11/943,924.
Claims priority of provisional application 60/866988, filed on Nov. 22, 2006.
Claims priority of provisional application 60/867263, filed on Nov. 27, 2006.
Prior Publication US 2008/0199870 A1, Aug. 21, 2008
Int. Cl. C12Q 1/68 (2006.01)

U.S. Cl. 435—6

39 Claims

1. A method of identifying an inhibitor of tRNA-target molecule binding, comprising: forming a mixture of a first nucleic acid molecule comprising a tRNA T.PSI.C-loop fragment, a target molecule capable of binding to the tRNA T.PSI.C-loop fragment, and a test compound, wherein the tRNA T.PSI.C-loop fragment contains at least one modified nucleotide; incubating the mixture under conditions that allow binding of the tRNA T.PSI.C-loop fragment and the target molecule in the absence of the test compound; and detecting whether or not the test compound inhibits the binding of the tRNA T.PSI.C-loop fragment and the target molecule, wherein the absence of binding of the tRNA fragment and the target molecule is indicative of the test compound being an inhibitor of tRNA-target molecule binding.