| US 7,575,901 B2 | ||
| Method for producing nucleotide by fermentation | ||
| Masahiro Kakehi, Kawasaki (Japan); Yoshihiro Usuda, Kawasaki (Japan); Yukiko Tabira, Kawasaki (Japan); and Shinichi Sugimoto, Kawasaki (Japan) | ||
| Assigned to Ajinomoto Co., Inc., Tokyo (Japan) | ||
| Filed on Mar. 12, 2004, as Appl. No. 10/798,339. | ||
| Application 10/798339 is a division of application No. 09/891287, filed on Jun. 27, 2001, abandoned. | ||
| Claims priority of application No. 2000-204260 (JP), filed on Jul. 05, 2000. | ||
| Prior Publication US 2004/0152171 A1, Aug. 05, 2004 | ||
| Int. Cl. C12P 19/38 (2006.01) | ||
| U.S. Cl. 435—87 [435/196; 435/252.33; 536/23.2] | 5 Claims |
| 1. A method for producing nucleoside 5′-phosphate ester, comprising the steps of culturing a bacterium belonging to Escherichia coli having an ability to produce nucleoside 5′-phosphate ester, in which expression of ushA gene and aphA gene is decreased as compared to a wild type strain by mutating or disrupting the ushA gene and the aphA gene, in a medium to produce and accumulate nucleoside 5′-phosphate ester in a medium, and collecting the nucleoside 5′-phosphate ester from the medium, wherein the nucleoside 5′-phosphate ester is selected from the group consisting of inosine 5′-phosphate ester and guanosine 5′-phosphate ester, and wherein the 5′-nucleotidase activity in the periplasm is substantially eliminated. |