	US 7,572,630 B2
	Method for differentiating primate embryonic stem cell into vascular cell
Kazuwa Nakao, Kyoto (Japan); Hiroshi Itoh, Kyoto (Japan); Jun Yamashita, Kyoto (Japan); Masakatsu Sone, Osaka (Japan); Yasushi Kondo, Osaka (Japan); and Yutaka Suzuki, Osaka (Japan)
Assigned to Mitsubishi Tanabe Pharma Corporation, Osaka-Shi (Japan)
Filed on Feb. 24, 2005, as Appl. No. 11/63,584.
Claims priority of provisional application 60/547809, filed on Feb. 27, 2004.
Prior Publication US 2005/0191744 A1, Sep. 01, 2005
Int. Cl. C12N 5/00 (2006.01); C12N 5/06 (2006.01); C12N 5/10 (2006.01)

U.S. Cl. 435—325 [435/347; 435/363; 435/373; 435/374; 435/375]

12 Claims

1. An in vitro method for differentiating a primate embryonic stem cell selected from CMK-6 cell strain or HES-3 cell strain into a vascular cell selected from the group consisting of a vascular endothelial cell, a mural cell and a vascular construct, comprising differentiating a primate embryonic stem cell into a vascular endothelial growth factor receptor (VEGFR-2)-positive cell, wherein the differentiated vascular cell lacks at least one of ALP, SSEA-3, SSEA-4, TRA1-60 and TRA1-81, wherein said differentiating comprises the steps of:

(I) co-culturing a primate embryonic stem cell with a feeder cell which includes OP9 cell strain, to differentiate into a cell population A comprising said vascular endothelial growth factor receptor (VEGFR-2)-positive and undifferentiated primate stem cell marker-negative cell, and

(II) separating substantially said VBGFR-2-positive and undifferentiated primate embryonic stem cell marker-negative cell from the cell population A obtained in the above step (I).