| US 7,572,574 B2 | ||
| Method of measuring neprilysin activity | ||
| Takaomi Saido, Saitama (Japan); Nobuhisa Iwata, Saitama (Japan); Satoshi Tsubuki, Saitama (Japan); Yoshie Takaki, Saitama (Japan); Takashi Saito, Saitama (Japan); and Tadashi Nakaya, Hokkaido (Japan) | ||
| Assigned to Riken, Saitama (Japan); and Takeda Pharmaceutical Company, Ltd, Osaka (Japan) | ||
| Appl. No. 10/512,588 PCT Filed Apr. 24, 2003, PCT No. PCT/JP03/05239 § 371(c)(1), (2), (4) Date Jul. 15, 2005, PCT Pub. No. WO03/091449, PCT Pub. Date Nov. 06, 2003. |
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| Claims priority of application No. 2002-126257 (JP), filed on Apr. 26, 2002; and application No. 2002-261250 (JP), filed on Sep. 06, 2002. | ||
| Prior Publication US 2006/0008842 A1, Jan. 12, 2006 | ||
| Int. Cl. C12Q 1/00 (2006.01); G01N 33/53 (2006.01); G01N 33/567 (2006.01); G01N 33/542 (2006.01); G01N 33/537 (2006.01) | ||
| U.S. Cl. 435—4 [435/7.1; 435/7.21; 435/7.8; 435/7.9; 435/7.92; 436/501] | 18 Claims |
| 1. A method for selecting a compound that enhances the activity of neprilysin which comprises:
incubating an isolated nerve cell comprising neprilysin with a test compound, a substrate of neprilysin, aminopeptidase, phosphoramidon,
and nitrosalicylaldehyde to form a fluorescent substance; and
measuring an increase in fluorescence in said cell relative to a control cell, wherein the increase in fluorescence identifies
a test compound that enhances neprilysin activity.
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