| US 7,566,573 B2 | ||
| Dual standard curve immunoassay | ||
| Charles R. Carpenter, Scarborough, Me. (US); Giosi Farace, Georgetown, Me. (US); Paul Scott MacHenry, Portland, Me. (US); Brian John Foster, Portland, Me. (US); Mirolee Blue Zieba, Westbrook, Me. (US); and Michael Ryan D'Angelo, Scarborough, Me. (US) | ||
| Assigned to Idexx Laboratories, Inc., Westbrook, Me. (US) | ||
| Filed on Dec. 19, 2005, as Appl. No. 11/311,567. | ||
| Prior Publication US 2007/0141717 A1, Jun. 21, 2007 | ||
| Int. Cl. G01N 33/541 (2006.01); G01N 33/533 (2006.01); G01N 33/543 (2006.01) | ||
| U.S. Cl. 436—540 [435/7.1; 436/518] | 7 Claims |
| 5. A method of determining the amount of an analyte in a sample over a broad range of potential analyte concentration, the
method comprising:
a. mixing the sample with a specific binding partner for the analyte conjugated to a chemiluminescent label and the specific
binding partner for the analyte conjugated to a fluorescent label,
b. measuring the amount of a signal generated from the fluorescent label that is associated with the analyte and the amount
of a signal from the chemiluminescent label that is associated with the analyte,
c. comparing the amount of the signal generated from the fluorescent label to a standard curve of the amount of the signal
from the fluorescent label versus known quantities of the analyte and comparing the amount of the signal generated from the
chemiluminescent label to a standard curve of the amount of signal from the chemiluminescent label versus known quantities
of analyte; and
d. determining the amount of the analyte in the sample from comparing step (c).
|