| US 7,566,544 B2 | ||
| Difference detection methods using matched multiple dyes | ||
| Jonathan Minden, Pittsburgh, Pa. (US); Alan Waggoner, Pittsburgh, Pa. (US); and Susan Janet Fowler, Buckinghamshire (United Kingdom) | ||
| Assigned to Carnegie Mellon University, Pittsburgh, Pa. (US) | ||
| Filed on May 01, 2002, as Appl. No. 10/137,180. | ||
| Application 10/137180 is a division of application No. 09/370743, filed on Aug. 09, 1999, granted, now 6,426,190. | ||
| Application 09/370743 is a continuation in part of application No. 08/425480, filed on Apr. 20, 1995, granted, now 6,127,134. | ||
| Prior Publication US 2002/0177122 A1, Nov. 28, 2002 | ||
| Int. Cl. G01N 33/567 (2006.01); C07K 1/28 (2006.01) | ||
| U.S. Cl. 435—7.2 [435/4; 435/6; 435/810; 436/63; 436/86; 436/172; 436/518; 436/800; 430/93; 204/459; 204/461] | 44 Claims |
| 1. A set of matched luminescent dyes comprising at least two different dyes which in use covalently bind to proteins within
an extract of proteins from at least two cell samples, wherein the dyes within said set:
(a) have a matched net charge which will maintain the overall net charge of the proteins upon such covalent binding and matched
ionic and pH characteristics whereby relative migration of a protein labeled with any one of said dyes is the same as relative
migration of said protein labeled with another dye in said set; and
(b) each emit luminescent light at a wavelength that is sufficiently different from the emitted luminescent light of remaining
dyes in said set to provide a detectably different light signal from the light signal of said remaining dyes in said set.
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