| US 7,566,538 B2 | ||
| Sequencing by incorporation | ||
| J. Wallace Parce, Palo Alto, Calif. (US); Theo T. Nikiforov, San Jose, Calif. (US); Tammy Burd Mehta, San Jose, Calif. (US); Anne R. Kopf-Sill, Portola Valley, Calif. (US); Andrea W. Chow, Los Altos, Calif. (US); and Michael R. Knapp, Redwood City, Calif. (US) | ||
| Assigned to Caliper Lifesciences Inc., Mountain View, Calif. (US) | ||
| Filed on Jan. 22, 2008, as Appl. No. 12/17,859. | ||
| Application 12/017859 is a division of application No. 11/460550, filed on Aug. 11, 2006, granted, now 7,344,865. | ||
| Application 10/413049 is a division of application No. 09/510205, filed on Feb. 22, 2000, granted, now 6,613,513, filed on Sep. 02, 2003. | ||
| Application 11/460550 is a continuation of application No. 10/413049, filed on Apr. 14, 2003, granted, now 7,105,300, filed on Sep. 12, 2006. | ||
| Claims priority of provisional application 60/128643, filed on Apr. 09, 1999. | ||
| Claims priority of provisional application 60/127825, filed on Apr. 05, 1999. | ||
| Claims priority of provisional application 60/121223, filed on Feb. 23, 1999. | ||
| Prior Publication US 2008/0131904 A1, Jun. 05, 2008 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. C12Q 1/68 (2006.01); C12P 19/34 (2006.01); C07H 21/00 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01) | ||
| U.S. Cl. 435—6 [435/91.1; 435/91.2; 536/23.1; 536/24.3; 536/24.33; 536/26.6] | 24 Claims |
| 1. A method of sequencing a nucleic acid, comprising:
(i) providing a first set of particles comprising at least one set of nucleic acid templates in a first microfluidic channel;
(ii) flowing a train of reagents comprising a plurality of sequencing reagents across the first set of particles, or flowing
the first set of particles through the reagent train, thereby sequentially contacting the at least one set of nucleic acid
templates with the plurality of sequencing reagents;
wherein one or more of the first set of particles and the reagent train is flowed through the microscale channel by one or
more of pressure, centripetal force, centrifugal force, a moving magnetic field, and an electrokinetic force;
(iii) detecting a signal resulting from exposure of the first set of particles to the reagent train, thereby providing a portion
of sequence of the nucleic acid template; and, repeating steps (ii) and (iii), thereby sequencing the nucleic acid.
|