| US 7,547,770 B2 | ||
| Colostral fractionation process | ||
| Andrew Keech, Scottsdale, Ariz. (US); and Rafael Jimenez-Flores, San Luis Obispo, Calif. (US) | ||
| Assigned to Advanced Protein Systems, Phoenix, Ariz. (US) | ||
| Filed on Nov. 03, 2004, as Appl. No. 10/981,152. | ||
| Claims priority of provisional application 60/516674, filed on Nov. 03, 2003. | ||
| Prior Publication US 2005/0092684 A1, May 05, 2005 | ||
| Int. Cl. C07K 14/00 (2006.01); A23J 1/20 (2006.01) | ||
| U.S. Cl. 530—417 [424/157.1] | 5 Claims |
| 1. A method for separating the proline-rich polypeptides essentially free of peptides and proteins of about 10,000 Daltons
or greater from the globular proteins having a molecular weight of between 10,000 and 500,000 Daltons in bovine colostrum,
the method comprising the steps of:
(a) subjecting at least a portion of a bovine colostrum sample to at least one separation procedure comprising an ion exchange
separation to yield separated proline-rich polypeptides essentially free of peptides and proteins of about 10,000 Daltons
or greater and separated globular proteins having a molecular weight of between 10,000 and 500,000 Daltons, wherein no more
than about 10% of the separated proline-rich polypeptides are denatured; and
(b) collecting the separated proline-rich polypeptides;
wherein at least 90% of the separated proline-rich polypeptides have a molecular weight of no more than about 2,500 Daltons.
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