| US 7,547,531 B2 | ||
| L-amino acid producing microorganism which has been modified to inactive the fimH gene, and a method for producing I-amino acid | ||
| Saori Kataoka, Kawasaki (Japan); Yuuta Nakai, Kawasaki (Japan); Takuji Ueda, Kawasaki (Japan); Yuji Joe, Kawasaki (Japan); and Hisao Ito, Kawasaki (Japan) | ||
| Assigned to Ajinomoto Co., Inc., Tokyo (Japan) | ||
| Filed on Jan. 17, 2006, as Appl. No. 11/275,562. | ||
| Claims priority of provisional application 60/644040, filed on Jan. 18, 2005. | ||
| Claims priority of provisional application 60/673338, filed on Apr. 21, 2005. | ||
| Claims priority of application No. 2005-009826 (JP), filed on Jan. 18, 2005; and application No. 2005-120222 (JP), filed on Apr. 18, 2005. | ||
| Prior Publication US 2006/0160191 A1, Jul. 20, 2006 | ||
| Int. Cl. C12P 13/04 (2006.01); C12P 13/14 (2006.01); C12P 13/08 (2006.01); C12N 15/00 (2006.01); C12N 1/21 (2006.01); C12P 21/00 (2006.01); C07K 14/00 (2006.01); C07H 21/00 (2006.01) | ||
| U.S. Cl. 435—106 [435/110; 435/115; 435/320.1; 435/69.1; 435/252.33; 536/23.1; 530/350] | 2 Claims |
| 1. A method for producing an L-amino acid, comprising
cultivating an Escherichia coli in a medium, and
collecting said L-amino acid from the medium,
wherein the fimH gene of said Escherichia coli has been inactivated by a method selected from the group consisting of
A) deleting the fimH gene,
B) modifying a promoter or a Shine-Dalgarno sequence of-the fimH gene,
C) introducing a missense mutation into the fimH gene,
D) introducing a nonsense mutation into the fimH gene, and
E) introducing a frame shift mutation into the fimH gene and wherein said fimH gene is selected from the group consisting
of:
(a) a DNA comprising the nucleotide sequence of SEQ ID NO:1, and
(b) a DNA that is able to hybridize with the complementary strand of the nucleotide of SEQ ID NO:1 under conditions comprising
washing at 60° C. and at a salt concentration corresponding to 1×SSC, 0.1 ×SDS, and wherein the DNA encodes fimH.
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