| US 7,518,726 B2 | ||
| Compact optical detection system for a microfluidic device | ||
| Aaron Rulison, Los Altos, Calif. (US); Jeffrey A. Wolk, Half Moon Bay, Calif. (US); Ernest C.W. Lee, Palo Alto, Calif. (US); Michael Slater, Modesto, Calif. (US); and Morten J. Jensen, San Francisco, Calif. (US) | ||
| Assigned to Caliper LifeSciences, Inc., Mountain View, Calif. (US) | ||
| Filed on Apr. 12, 2006, as Appl. No. 11/404,043. | ||
| Claims priority of provisional application 60/670736, filed on Apr. 12, 2005. | ||
| Prior Publication US 2006/0227325 A1, Oct. 12, 2006 | ||
| Int. Cl. G01B 11/00 (2006.01) | ||
| U.S. Cl. 356—401 [356/246] | 20 Claims |
| 5. An optical detection system for a microfluidic device, comprising:
a microfluidic device comprising:
a first substrate;
a plurality of channels formed in the first substrate, wherein at least one channel is a microfluidic channel;
a plurality of optical alignment marks formed in the first substrate spaced apart from the plurality of channels, each alignment
mark having a curved wall;
a second substrate bonded to the first substrate such that the channels are covered and the alignment marks are closed;
wherein at least one channel is positioned between at least two alignment marks;
a light-emitting diode;
means for collimating light emitted by the light-emitting diode;
an aspherical, fused-silica objective lens;
means for directing the collimated light through the objective lens onto the microfluidic device; and
means for detecting a fluorescent signal emitted from the microfluidic device.
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