US 7,517,661 B2
	Methods of screening for ligands for FPRL2
Makoto Kobayashi, Osaka (Japan); Yugo Habata, Tsukuba (Japan); Ryo Fujii, Tsukuba (Japan); and Shuji Hinuma, Tsukuba (Japan)
Assigned to Takeda Pharmaceutical Company Limited, Osaka (Japan)
Appl. No. 10/554,234 PCT Filed Apr. 22, 2004, PCT No. PCT/JP2004/005829 § 371(c)(1), (2), (4) Date Oct. 21, 2005, PCT Pub. No. WO2004/095023, PCT Pub. Date Nov. 04, 2004.
Claims priority of application No. 2003-118760 (JP), filed on Apr. 23, 2003.
Prior Publication US 2007/0009513 A1, Jan. 11, 2007
Int. Cl. G01N 33/567 (2006.01); C07K 14/705 (2006.01)

U.S. Cl. 435—7.21  [436/501; 530/350]

10 Claims

1. A method of screening for a compound or a salt thereof, that changes the binding property of a G protein-coupled receptor protein comprising the amino acid sequence represented by SEQ ID NO: 1, or a salt thereof, to a ligand peptide comprising the amino acid sequence represented by SEQ ID NO:6, its amide, or a salt thereof, the method comprising: contacting said receptor protein, or a salt thereof, with said ligand peptide, its amide, or a salt thereof, and determining a level of binding by said ligand peptide, its amide, or salt thereof, to said receptor protein or salt thereof, in the presence of said compound or salt thereof, and in the absence of said compound or salt thereof, wherein a difference in binding levels by said ligand peptide, its amide or salt thereof, to said receptor protein or salt thereof, in the presence of said compound or salt thereof, compared to in the absence of said compound or salt thereof, indicates that the compound changes the binding property of said receptor protein to said ligand peptide.