| US 7,517,538 B2 | ||
| Solid supports functionalised with phosphorus dendrimers, method for preparing same and uses thereof | ||
| Emmanuelle Trevisiol, Cornebarrieu (France); Julien Leclaire, Toulouse (France); Geneviève Pratviel, Toulouse (France); Anne-Marie Caminade, Toulouse (France); Jean Francois, Castenet (France); Jean-Pierre Majoral, Ramonville (France); and Bernard Meunier, Castenet (France) | ||
| Assigned to Centre National de la Recherche Scientifique, (France); and Institut National de la Recherche Agronomique, (France) | ||
| Appl. No. 10/512,133 PCT Filed Apr. 17, 2003, PCT No. PCT/FR03/01231 § 371(c)(1), (2), (4) Date May 25, 2005, PCT Pub. No. WO03/091304, PCT Pub. Date Nov. 06, 2003. |
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| Claims priority of application No. 02 05049 (FR), filed on Apr. 23, 2002. | ||
| Prior Publication US 2005/0214767 A1, Sep. 29, 2005 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. A61K 9/14 (2006.01); C12Q 1/68 (2006.01); C07H 21/00 (2006.01); A61K 43/04 (2006.01); G01N 15/06 (2006.01) | ||
| U.S. Cl. 424—486 [435/6; 435/455; 536/25.3; 422/68.1; 514/44] | 15 Claims |
| 1. A solid support, comprising at least one surface covalently functionalized with phosphorus-containing dendrimers having
a central core that contains at least two functional groups and comprising at their periphery several functions binding said
dendrimers to said surface and also allowing the binding or the in situ synthesis of molecules of interest, said dendrimers
being between 1 and 20 nm in size, characterized in that the dendrimers are chosen from those consisting of:
a central layer in the form of a central core P0, optionally containing phosphorus, comprising from 2 to 12 functionalized groups,
n intermediate layers, which may be identical or different, each of said intermediate layers consisting of P1 units corresponding to formula (I) below:
 in which:
L is an oxygen, phosphorus, sulfur or nitrogen atom,
M represents one of the following groups:
an aromatic group di-, tri- or tetrasubstituted with alkyl groups, alkoxy groups, unsaturated groups of the C1-C12 olefinic, azo or acetylenic type, all these groups possibly incorporating phosphorus, oxygen, nitrogen or sulfur atoms or
halogens, or
an alkyl or alkoxy group comprising several substituents as defined when M is an aromatic group,
R1 and R2, which may be identical or different, represent a hydrogen atom or one of the following groups: alkyl, alkoxy, aryl, optionally
comprising phosphorus, oxygen, sulfur or nitrogen atoms or halogens with R2 usually being different than R1,
n is an integer between 1 and 11,
E is an oxygen, sulfur or nitrogen atom, said nitrogen atom possibly being linked to an alkyl, alkoxy or aryl group, all these
groups possibly incorporating phosphorus, oxygen, nitrogen or sulfur atoms or halogens,
an external layer consisting of units P2, which may be identical or different, and corresponding to formula II below:
 in which:
W represents one of the following groups: alkyl, alkoxy, aryl, all these groups possibly comprising phosphorus, oxygen, nitrogen
or sulfur atoms or halogens,
X represents an aldehyde, thiol, amine, epoxide, carboxylic acid, alcohol or phenol group.
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