| US 7,517,521 B2 | ||
| Method of isolating human neuroepithelial precursor cells from human fetal tissue | ||
| Margot Mayer-Proschel, Pittsford, N.Y. (US); Mahendra S. Rao, Salt Lake City, Utah (US); Patrick A. Tresco, Sandy, Utah (US); and Darin J. Messina, Salt Lake City, Utah (US) | ||
| Assigned to University of Utah Research Foundation, Salt Lake City, Utah (US) | ||
| Filed on Jan. 14, 2005, as Appl. No. 11/36,004. | ||
| Application 11/036004 is a division of application No. 09/813429, filed on Mar. 21, 2001, granted, now 6,852,532. | ||
| Prior Publication US 2005/0125848 A1, Jun. 09, 2005 | ||
| Int. Cl. A61K 48/00 (2006.01); C12N 5/08 (2006.01) | ||
| U.S. Cl. 424—93.7 [435/375; 435/368] | 1 Claim |
| 1. A method for transplanting an isolated population of human neuroepithelial precursor cells into an animal comprising:
(a) isolating human neuroepithelial precursor cells from human fetal tissue by a method which comprises
(i) culturing human fetal cells in medium containing fibroblast growth factor and chick embryo extract; and
(ii) immunodepleting from the cultured human fetal cells any cells expressing A2B5, NG2 and eNCAM so that an isolated population
of human neuroepithelial precursor cells remains; and
(b) transplanting the isolated human neuroepithelial precursor cells into the central nervous system of an animal, wherein
said animal is immunosuppressed or is a human.
|