US 7,488,816 B2
	Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same
Jean-Luc Jestin, Paris (France); Sophie Vichier-Guerre, La Celle-Saint Cloud (France); and Stephane Ferris, Mennecy (France)
Assigned to Institut Pasteur, Paris (France)
Filed on Feb. 25, 2005, as Appl. No. 11/65,943.
Application 11/065943 is a continuation in part of application No. 10/787219, filed on Feb. 27, 2004.
Prior Publication US 2005/0250131 A1, Nov. 10, 2005
This patent is subject to a terminal disclaimer.
Int. Cl. C07H 21/04 (2006.01)

U.S. Cl. 536—24.3  [536/24.32; 536/24.33]

31 Claims

1. A purified polynucleotide which encodes a thermostable polypeptide comprising an amino acid sequence having at least 85% identity to residues 13-555 of SEQ ID NO: 26, wherein said polypeptide has (a) a mutation at E465, (b) a mutation at M470 wherein said mutation at M470 is not M470R, or (c) a mutation at M470 and a mutation at E465.