| US 7,482,444 B2 | ||
| Terminating substrates for DNA polymerases | ||
| Jari Hovinen, Raisio (Finland) | ||
| Assigned to Wallac Oy, Turku (Finland) | ||
| Filed on Feb. 26, 2007, as Appl. No. 11/710,470. | ||
| Claims priority of provisional application 60/781380, filed on Mar. 13, 2006. | ||
| Prior Publication US 2007/0248973 A1, Oct. 25, 2007 | ||
| Int. Cl. C07H 19/04 (2006.01); C07H 21/00 (2006.01); C07H 21/02 (2006.01); C12P 19/34 (2006.01); C12Q 1/68 (2006.01) | ||
| U.S. Cl. 536—26.6 [536/23.1; 536/24.3; 536/24.33; 435/6; 435/91.1] | 9 Claims |
| 1. A molecule of formula (I)
Z-R-L-X (I)
wherein,
Z is a triphosphate anion or its organic or inorganic salt;
R is a recognizing moiety, which is a nucleoside comprising a base bound to a 2,3-dideoxyribose or its acyclic derivative;
and
Z-R has the formula (II)
 wherein the ribose ring optionally is replaced by an acyclic derivative in which one or both of the 2- and 3-carbon atoms
from the ribose ring optionally are missing;
-L- is a linker; and
X is a non-luminescent lanthanide(III) chelate comprising a lanthanide(III) ion, wherein the lanthanide(III) ion is selected
from the group consisting of europium, terbium,dysprosium and samarium.
|