US 7,473,680 B2
Remodeling and glycoconjugation of peptides
Shawn DeFrees, North Wales, Pa. (US); David A. Zopf, Wayne, Pa. (US); Robert J. Bayer, San Diego, Calif. (US); David James Hakes, Willow Grove, Pa. (US); Caryn Bowe, Doylestown, Pa. (US); and Xi Chen, Lansdale, Pa. (US)
Assigned to Neose Technologies, Inc., Horsham, Pa. (US)
Filed on Jul. 15, 2005, as Appl. No. 11/183,205.
Application 11/183205 is a division of application No. 10/287994, granted, now 7,138,371, previously published as PCT/US02/32263, filed on Oct. 09, 2002.
Claims priority of provisional application 60/407527, filed on Aug. 28, 2002.
Claims priority of provisional application 60/404249, filed on Aug. 16, 2002.
Claims priority of provisional application 60/396594, filed on Jul. 17, 2002.
Claims priority of provisional application 60/391777, filed on Jun. 25, 2002.
Claims priority of provisional application 60/387292, filed on Jun. 07, 2002.
Claims priority of provisional application 60/334301, filed on Nov. 28, 2001.
Claims priority of provisional application 60/334233, filed on Nov. 28, 2001.
Prior Publication US 2006/0030521 A1, Feb. 09, 2006
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 38/00 (2006.01)
U.S. Cl. 514—8 43 Claims
 
1. A method of forming a covalent conjugate between a water soluble polymer and a glycosylated or non-glycosylated peptide, wherein said water soluble polymer is conjugated to said peptide via an intact glycosyl linking group interposed between and covalently linked to both said peptide and said water soluble polymer, said method comprising:
contacting said peptide with a mixture comprising a nucleotide sugar covalently linked to said water soluble polymer and a glycosyltransferase for which said nucleotide sugar is a substrate under conditions sufficient to form said conjugate.