US 7,473,561 B2
Bone morphogenetic protein-2 in the treatment and diagnosis of cancer
John Langenfeld, Flemington, N.J. (US)
Assigned to University of Medicine and Dentistry of New Jersey, Somerset, N.J. (US)
Filed on May 17, 2006, as Appl. No. 11/435,852.
Application 11/435852 is a division of application No. 10/692824, filed on Oct. 23, 2003, abandoned.
Application 10/692824 is a continuation in part of application No. 10/044716, filed on Jan. 11, 2002, abandoned.
Claims priority of provisional application 60/261252, filed on Jan. 12, 2001.
Prior Publication US 2006/0198844 A1, Sep. 07, 2006
Int. Cl. G01N 33/566 (2006.01)
U.S. Cl. 436—501  [530/350; 435/4] 1 Claim
 
1. A method for identifying a candidate agent for use in reducing vascularization in non-small cell lung tumors comprising:
(a) contacting bone morphogenetic protein-2 (BMP-2) and BMP-2 receptor IB in the presence of a test agent under conditions suitable to permit the formation of a BMP-2/BMP-2 receptor complex, wherein said BMP-2 comprises the amino acid sequence of amino acids 283-396 of SEQ ID NO: 2 and wherein said BMP-2 receptor IB comprises the amino acid sequence of amino acids 14-502 of SEQ ID NO: 14;
(b) determining the amount of the BMP-2/BMP-2 receptor complex formed in the presence of said test agent; and
(c) determining that said test agent is a candidate agent for use in reducing vascularization in non-small cell lung tumors if the amount of the BMP-2/BMP-2 receptor complex formed in the presence of said test agent is lower than the amount of the BMP-2/BMP-2 receptor complex formed in the absence of said test agent,
thereby identifying a candidate agent for use in reducing vascularization in non-small cell lung tumors.