| US 7,473,558 B2 | ||
| Marker useful for detection and measurement of free radical damage and method | ||
| David Bar-Or, Englewood, Colo. (US); and Edward Lau, Boulder, Colo. (US) | ||
| Assigned to Ischemia Technologies, Inc., Waltham, Mass. (US) | ||
| Filed on Aug. 30, 2002, as Appl. No. 10/232,341. | ||
| Application 10/232341 is a division of application No. 09/165961, filed on Oct. 02, 1998, granted, now 6,475,743. | ||
| Prior Publication US 2003/0017506 A1, Jan. 23, 2003 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. G01N 33/20 (2006.01) | ||
| U.S. Cl. 436—73 [436/63; 436/74; 436/79; 436/80; 436/81; 436/82; 436/83; 436/84; 436/182] | 10 Claims |
| 1. A method for detecting or quantifying free radical damage comprising:
receiving a biological sample containing albumin, including an albumin having an unmodified N-terminus which is able to bind
metal ions and, possibly, a modified albumin having a reduced ability to bind metal ions at its N-terminus; and
determining the presence of modified albumin present in the biological sample by:
a) contacting the biological sample with an excess quantity of a metal ion, the metal ion being capable of binding to the
unmodified N-terminus of albumin, to form a mixture of bound metal ions and unbound metal ions; and
b) detecting the presence and quantity of bound metal ions, the presence and quantity of bound metal ions being derminative
of the existence and concentration of the modified albumin present in the biological sample, and the existence and concentration
of the modified albumin in the biological sample being indicative of the presence and amount of free radical damage.
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