| US 7,473,419 B2 | ||
| Intravascular delivery of nucleic acid | ||
| Jon A. Wolff, Madison, Wis. (US); James E. Hagstrom, Middleton, Wis. (US); Hans Herweijer, Madison, Wis. (US); Vladimir G. Budker, Middleton, Wis. (US); and Julia Hegge, Monona, Wis. (US) | ||
| Assigned to Roche Madison Inc., Madison, Wis. (US) | ||
| Filed on Nov. 07, 2005, as Appl. No. 11/268,276. | ||
| Application 11/268276 is a division of application No. 11/008856, filed on Dec. 10, 2004, granted, now 7,015,040. | ||
| Application 11/008856 is a continuation in part of application No. 10/855175, filed on May 27, 2004. | ||
| Application 10/855175 is a continuation in part of application No. 10/085378, filed on Feb. 27, 2002, granted, now 6,897,068. | ||
| Application 10/085378 is a continuation in part of application No. 09/707000, filed on Nov. 06, 2000. | ||
| Application 10/085378 is a continuation in part of application No. 09/450315, filed on Nov. 29, 1999, granted, now 6,379,966. | ||
| Claims priority of provisional application 60/121730, filed on Feb. 26, 1999. | ||
| Claims priority of provisional application 60/146564, filed on Jul. 30, 1999. | ||
| Claims priority of provisional application 60/163719, filed on Nov. 05, 1999. | ||
| Claims priority of provisional application 60/473654, filed on May 28, 2003. | ||
| Claims priority of provisional application 60/500211, filed on Sep. 04, 2003. | ||
| Prior Publication US 2006/0093584 A1, May 04, 2006 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. A01N 63/00 (2006.01); A61K 48/00 (2006.01) | ||
| U.S. Cl. 424—93.2 [435/455; 435/456; 514/44] | 36 Claims |
| 1. An in vivo process for delivering a nucleic acid to an extravascular cell in a limb of a mammal, comprising:
a) forming a complex containing the nucleic acid in a pharmaceutically acceptable solution;
b) inserting an injection devise into a vessel in the limb;
c) applying a device to occlude blood flow externally around the limb;
d) occluding blood flow to an from the limb; and,
e) injecting the solution through the injector into the vessel thereby increasing permeability of the blood vessel, passing
the nucleic acid though the blood vessel and delivering the nucleic acid to the extravascular cell.
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